HUNK antibody (AA 220-300)

Catalog No. ABIN7221478

This Rabbit Polyclonal antibody specifically detects HUNK in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for HUNK antibody (AA 220-300) (ABIN7221478)

Target: HUNK (Hormonally Up-Regulated Neu-Associated Kinase (HUNK))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HUNK antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-HUNK Antibody

Binding Specificity: AA 220-300

Purpose: HUNK Polyclonal Antibody

Specificity: HUNK Polyclonal Antibody detects endogenous levels of HUNK protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human HUNK at AA range: 220-300

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for HUNK

Target: HUNK (Hormonally Up-Regulated Neu-Associated Kinase (HUNK))

Alternative Name: HUNK

Background: Rabbit Anti-HUNK Polyclonal Antibody,HUNK, MAKV, Hormonally up-regulated neu tumor-associated kinase, B19, Serine/threonine-protein kinase MAK-V,The HUNK (hormonally upregulated Neu-associated kinase) protein, also designated MAK-V in mouse, has been identified as a novel SNF1-related serine/threonine kinase. The human HUNK gene localizes to chromosome 21q22 and encodes a protein with nucleocytoplasmic distribution and localizes to the centrosome. Overexpression of the HUNK protein associates with approximately 50 % of breast carcinomas, and may provide diagnostic-prognostic value as a molecular marker. Serine/threonine-protein kinase SNF1-like kinase 2 (SIK) phosphorylates Ser-794 of IRS1 in insulin-stimulated adipocytes, which may modulate the efficiency of insulin signal transduction. SIK is activated by phosphorylation on Thr-175 by STK11 in complex with STE20-related adapter-alpha and CAB39.,Hormonally up-regulated neu tumor-associated kinase

Molecular Weight: observerd band 75kDa

Gene ID: 30811

UniProt: P57058