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HUNK antibody (Kinase Domain)

HUNK Reactivity: Human, Mouse, Rat, Monkey, Cow, Chicken, Dog, Hamster, Horse, Rabbit IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN1048971
  • Target See all HUNK products
    HUNK (Hormonally Up-Regulated Neu-Associated Kinase (HUNK))
    Binding Specificity
    • 15
    • 9
    • 9
    • 8
    • 7
    • 6
    • 6
    • 3
    • 1
    • 1
    • 1
    • 1
    Kinase Domain
    Reactivity
    Human, Mouse, Rat, Monkey, Cow, Chicken, Dog, Hamster, Horse, Rabbit
    Host
    • 52
    • 3
    Rabbit
    Clonality
    • 53
    • 2
    Polyclonal
    Conjugate
    • 21
    • 5
    • 5
    • 5
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This HUNK antibody is un-conjugated
    Application
    • 51
    • 37
    • 16
    • 13
    • 13
    • 10
    • 3
    • 3
    • 2
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Brand
    IHC-plus™
    Specificity
    Human HUNK. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Predicted Reactivity
    Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Horse, Rabbit, Turkey, Chicken, Lizard (100%) Opossum, Xenopus (94%) Pufferfish (83%).
    Purification
    Immunoaffinity purified
    Immunogen
    Synthetic 18 amino acid peptide from Protein kinase domain of human HUNK. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Horse, Rabbit, Turkey, Chicken, Lizard (100%), Opossum, Xenopus (94%), Pufferfish (83%).

    Type of Immunogen: Synthetic peptide
  • Application Notes
    Approved: IHC, IHC-P (8 μg/mL)
    Comment

    Target Species of Antibody: Human

    Assay Procedure

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    HUNK (Hormonally Up-Regulated Neu-Associated Kinase (HUNK))
    Alternative Name
    HUNK / B19 (HUNK Products)
    Synonyms
    HUNK antibody, TPT1P antibody, Bstk1 antibody, Mak-v antibody, RGD1565091 antibody, hormonally up-regulated Neu-associated kinase antibody, hormonally upregulated Neu-associated kinase antibody, HUNK antibody, Hunk antibody
    Background
    Name/Gene ID: HUNK
    Subfamily: AMPK/SNF1
    Family: Protein Kinase

    Synonyms: HUNK, B19, Bstk1, Mak-v, MAKV
    Gene ID
    30811
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