HuB/D (AA 30-110) antibody

Catalog No. ABIN7220210

The Rabbit Polyclonal anti-HuB/D antibody is suitable to detect HuB/D in samples from Human, Mouse and Rat. It has been validated for ELISA, IF, WB and IHC (p).

Quick Overview for HuB/D (AA 30-110) antibody (ABIN7220210)

Target: HuB/D

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details

Binding Specificity: AA 30-110

Purpose: HuB/D Polyclonal Antibody

Specificity: HuB/D Polyclonal Antibody detects endogenous levels of HuB/D protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human HuB/D at AA range: 30-110

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details

Target: HuB/D

Background: Rabbit Anti-HuB/D Polyclonal Antibody,ELAVL2, HUB, ELAV-like protein 2, ELAV-like neuronal protein 1, Hu-antigen B, HuB, Nervous system-specific RNA-binding protein Hel-N1, ELAVL4, HUD, PNEM, ELAV-like protein 4, Hu-antigen D, HuD, Paraneoplastic encephalomyelitis antigen HuD,Eukaryotic translation initiation factor-5 (EIF5) interacts with the 40S initiation complex to promote hydrolysis of bound GTP with concomitant joining of the 60S ribosomal subunit to the 40S initiation complex. The resulting functional 80S ribosomal initiation complex is then active in peptidyl transfer and chain elongations (summary by Si et al. 1996 [PubMed 8663286]).,ELAV-like protein 2

Molecular Weight: observerd band 40kDa

Gene ID: 1993, 1996