GPR109B antibody (AA 270-350)

Catalog No. ABIN7225678

This Rabbit Polyclonal antibody specifically detects GPR109B in WB, ELISA and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for GPR109B antibody (AA 270-350) (ABIN7225678)

Target: GPR109B (HCAR3) (Hydroxycarboxylic Acid Receptor 3 (HCAR3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR109B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-GPR109B Antibody

Binding Specificity: AA 270-350

Purpose: HM74 Polyclonal Antibody

Specificity: HM74 Polyclonal Antibody detects endogenous levels of HM74 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the C-terminal region of human HM74 at AA range: 270-350

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IF (1:200-1:1000), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for GPR109B

Target: GPR109B (HCAR3) (Hydroxycarboxylic Acid Receptor 3 (HCAR3))

Alternative Name: HM74

Background: Rabbit Anti-HM74 Polyclonal Antibody,HCAR3, GPR109B, HCA3, HM74B, NIACR2, Hydroxycarboxylic acid receptor 3, G-protein coupled receptor 109B, G-protein coupled receptor HM74, G-protein coupled receptor HM74B, Niacin receptor 2, Nicotinic acid receptor 2, HCAR2, GPR109A, HCA2,HM74, also known as PUMAG or Puma-g, is a member of the G protein coupled receptor (GPCR) superfamily. In humans, HM74 is encoded by two different genes (GPR109A and GPR109B) that express two distinct proteins, namely HM74A and HM74B (also known as simply HM74), which are 96 % homologous. In mice and rats, only one gene (deisngnated Gpr109a) encodes the HM74 protein. HM74B is a Gi protein-coupled receptor that mediates the metabolic effects of nicotinic acid. Localizing to the cell membrane, HM74B is highly expressed in adipocytes, immune cells and spleen and, like all members of the GPCR superfamily, contains seven transmembrane domains. HM74B lacks the characteristic N-linked glycosylation sites that are present in other GPCR family members and also shows a more diverged amino acid sequence homology from most family members, implying different ligand specificity.,G-protein coupled receptor 109B

Molecular Weight: observerd band 45kDa

Gene ID: 8843, 338442