GPR109B antibody (Cytoplasmic Domain)

Catalog No. ABIN1048689

Product Details anti-GPR109B Antibody

Target: GPR109B (HCAR3) (Hydroxycarboxylic Acid Receptor 3 (HCAR3))

Binding Specificity: Cytoplasmic Domain

Reactivity: Human, Dog, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR109B antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Brand: IHC-plus™

Specificity: Human GPR109B / HM74. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except HCAR2 (100 % ), GPR81 (50 %).

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla, Dog, Hamster, Panda (100%) Mouse, Horse, Guinea pig (95%) Rat (90%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 20 amino acid peptide from 3rd cytoplasmic domain of human GPR109B / HM74. Percent identity with other species by BLAST analysis: Human, Gorilla, Dog, Hamster, Panda (100%), Mouse, Horse, Guinea pig (95%), Rat (90%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ICC, IHC, IHC-P (19 - 38 μg/mL)

Comment:

Target Species of Antibody: Human

Assay Procedure:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for GPR109B

Target: GPR109B (HCAR3) (Hydroxycarboxylic Acid Receptor 3 (HCAR3))

Alternative Name: HCAR3 / GPR109B / HM74 (HCAR3 Products)

Synonyms: GPR109B antibody, HCA3 antibody, HM74 antibody, PUMAG antibody, Puma-g antibody, NIACR2 antibody, hm74 antibody, niacr2 antibody, hydroxycarboxylic acid receptor 3 antibody, HCAR3 antibody, hcar3 antibody

Background: Name/Gene ID: HCAR3
Subfamily: Nicotinic Acid
Family: GPCR

Synonyms: HCAR3, GPR109B, GTP-binding protein, NIACR2, HM74, HM74b, Nicotinic acid receptor 2, Putative chemokine receptor, Niacin receptor 2, Puma-g, HCA3, PUMAG

Gene ID: 8843