IL4 Receptor antibody (pTyr497)

Catalog No. ABIN7221770

This Rabbit Polyclonal antibody specifically detects IL4 Receptor in WB, ELISA, IF and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for IL4 Receptor antibody (pTyr497) (ABIN7221770)

Target: IL4 Receptor (IL4R) (Interleukin 4 Receptor (IL4R))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL4 Receptor antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-IL4 Receptor Antibody

Binding Specificity: pTyr497

Purpose: IL-4Rα (phospho Tyr497) Polyclonal Antibody

Specificity: Phospho-IL-4Rα (Y497) Polyclonal Antibody detects endogenous levels of IL-4Rα protein only when phosphorylated at Y497.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human IL-4Ralpha Phospho-Tyr497

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for IL4 Receptor

Target: IL4 Receptor (IL4R) (Interleukin 4 Receptor (IL4R))

Alternative Name: IL-4Ralpha

Background: Rabbit Anti-IL-4Rα (phospho Tyr497) Polyclonal Antibody,IL4R, IL4RA, 582J2.1, Interleukin-4 receptor subunit alpha, IL-4 receptor subunit alpha, IL-4R subunit alpha, IL-4R-alpha, IL-4RA, CD antigen CD124,IL4R encodes the alpha chain of the interleukin-4 receptor, a type I transmembrane protein that can bind interleukin 4 and interleukin 13 to regulate IgE production. The encoded protein also can bind interleukin 4 to promote differentiation of Th2 cells. A soluble form of the encoded protein can be produced by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. Allelic variations in this gene have been associated with atopy, a condition that can manifest itself as allergic rhinitis, sinusitus, asthma, or eczema. Polymorphisms in this gene are also associated with resistance to human immunodeficiency virus type-1 infection. Alternate splicing results in multiple transcript variants.,Interleukin-4 receptor subunit alpha

Molecular Weight: observerd band 90kDa

Gene ID: 3566

UniProt: P24394

Pathways: JAK-STAT Signaling, Positive Regulation of Immune Effector Process