LIM Domain Kinase 1 antibody (pThr508)

Catalog No. ABIN7213028

The Rabbit Polyclonal anti-LIM Domain Kinase 1 antibody has been validated for WB, ELISA, IF and IHC (p). It is suitable to detect LIM Domain Kinase 1 in samples from Human, Mouse and Rat.

Quick Overview for LIM Domain Kinase 1 antibody (pThr508) (ABIN7213028)

Target: LIM Domain Kinase 1 (LIMK1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIM Domain Kinase 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-LIM Domain Kinase 1 Antibody

Binding Specificity: pThr508

Purpose: LIMK-1 (phospho Thr508) Polyclonal Antibody

Specificity: Phospho-LIMK-1 (T508) Polyclonal Antibody detects endogenous levels of LIMK-1 protein only when phosphorylated at T508.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human LIMK-1 Phospho-Thr508

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for LIM Domain Kinase 1

Target: LIM Domain Kinase 1 (LIMK1)

Alternative Name: LIMK-1

Background: Rabbit Anti-LIMK-1 (phospho Thr508) Polyclonal Antibody,LIMK1, LIMK, LIM domain kinase 1, LIMK-1,There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. LIM domain kinase 1 is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. LIM domain kinase 1 also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.,LIM domain kinase 1

Molecular Weight: observerd band 72kDa

Gene ID: 3984

UniProt: P53667

Pathways: Caspase Cascade in Apoptosis, Regulation of Cell Size, CXCR4-mediated Signaling Events