LIMK2 antibody (pThr505)

Catalog No. ABIN7213030

The Rabbit Polyclonal anti-LIMK2 antibody is suitable to detect LIMK2 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IF and IHC (p).

Quick Overview for LIMK2 antibody (pThr505) (ABIN7213030)

Target: LIMK2 (LIM Domain Kinase 2 (LIMK2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIMK2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-LIMK2 Antibody

Binding Specificity: pThr505

Purpose: LIMK-2 (phospho Thr505) Polyclonal Antibody

Specificity: Phospho-LIMK-2 (T505) Polyclonal Antibody detects endogenous levels of LIMK-2 protein only when phosphorylated at T505.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human LIMK-2 Phospho-Thr505

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for LIMK2

Target: LIMK2 (LIM Domain Kinase 2 (LIMK2))

Alternative Name: LIMK-2

Background: Rabbit Anti-LIMK-2 (phospho Thr505) Polyclonal Antibody,LIMK2, LIM domain kinase 2, LIMK-2,There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIM domain kinase 2 encoded by LIMK2 is phosphorylated and activated by ROCK, a downstream effector of Rho, and LIM domain kinase 2, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for LIMK2.,LIM domain kinase 2

Gene ID: 3985

UniProt: P53671