MED28 antibody (AA 20-100)

Catalog No. ABIN7229628

This anti-MED28 antibody is a Rabbit Polyclonal antibody detecting MED28 in ELISA and WB. Suitable for Human, Mouse and Rat.

Quick Overview for MED28 antibody (AA 20-100) (ABIN7229628)

Target: MED28 (Mediator Complex Subunit 28 (MED28))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED28 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details anti-MED28 Antibody

Binding Specificity: AA 20-100

Purpose: MED28 Polyclonal Antibody

Specificity: The antibody detects endogenous levels of MED28 protein

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen

Immunogen: Synthesized peptide derived from part region of human MED28 protein at AA range: 20-100

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:5000-1:20000).

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium Azide as preservative and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for MED28

Target: MED28 (Mediator Complex Subunit 28 (MED28))

Alternative Name: MED28

Background: Rabbit Anti-MED28 Polyclonal Antibody,Mediator of RNA polymerase II transcription subunit 28, Endothelial-derived protein 1, Mediator complex subunit 28, Merlin and Grb2-interacting cytoskeletal protein, Magicin, Tumor angiogenesis marker EG-1,MED28 (Mediator Complex Subunit 28) is a Protein Coding gene. Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May be part of a complex containing NF2/merlin that participates in cellular signaling to the actin cytoskeleton downstream of tyrosine kinase signaling pathways.,MED28

Molecular Weight: observerd band 19kDa

Gene ID: 80306

UniProt: Q9H204

Pathways: Stem Cell Maintenance, Regulation of Muscle Cell Differentiation