MRPL32 antibody (AA 70-150)

Catalog No. ABIN7224340

This Rabbit Polyclonal antibody specifically detects MRPL32 in WB, ELISA, IF and IHC (p). It exhibits reactivity toward Human.

Quick Overview for MRPL32 antibody (AA 70-150) (ABIN7224340)

Target: MRPL32 (Mitochondrial Ribosomal Protein L32 (MRPL32))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MRPL32 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MRPL32 Antibody

Binding Specificity: AA 70-150

Purpose: MRP-L32 Polyclonal Antibody

Specificity: MRP-L32 Polyclonal Antibody detects endogenous levels of MRP-L32 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human MRP-L32 at AA range: 70-150

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for MRPL32

Target: MRPL32 (Mitochondrial Ribosomal Protein L32 (MRPL32))

Alternative Name: MRP-L32

Background: Rabbit Anti-MRP-L32 Polyclonal Antibody,MRPL32, HSPC283, 39S ribosomal protein L32, mitochondrial, L32mt, MRP-L32,Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. MRPL32 (mitochondrial ribosomal protein L32) encodes a 39S subunit protein that belongs to the L32 ribosomal protein family. A pseudogene corresponding to MRPL32 is found on chromosome Xp.,39S ribosomal protein L32 mitochondrial

Molecular Weight: observerd band 21kDa

Gene ID: 64983

UniProt: Q9BYC8