MRPS35 antibody (AA 180-260)

Catalog No. ABIN7215880

This Rabbit Polyclonal antibody specifically detects MRPS35 in WB, ELISA and IHC (p). It exhibits reactivity toward Human.

Quick Overview for MRPS35 antibody (AA 180-260) (ABIN7215880)

Target: MRPS35 (Mitochondrial Ribosomal Protein S35 (MRPS35))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MRPS35 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MRPS35 Antibody

Binding Specificity: AA 180-260

Purpose: MRP-S35 Polyclonal Antibody

Specificity: MRP-S35 Polyclonal Antibody detects endogenous levels of MRP-S35 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human MRP-S35 at AA range: 180-260

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for MRPS35

Target: MRPS35 (Mitochondrial Ribosomal Protein S35 (MRPS35))

Alternative Name: MRP-S35

Background: Rabbit Anti-MRP-S35 Polyclonal Antibody,MRPS35, MRPS28, HDCMD11P, MDS023, PSEC0213, 28S ribosomal protein S35, mitochondrial, MRP-S35, S35mt, 28S ribosomal protein S28, mitochondrial, MRP-S28, S28mt,28S ribosomal protein S35 mitochondrial (mitochondrial ribosomal protein S35) is encoded by nuclear genes MRPS35 and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that has had confusing nomenclature in the literature. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Pseudogenes corresponding to this gene are found on chromosomes 3p, 5q, and 10q.,28S ribosomal protein S35 mitochondrial

Molecular Weight: observerd band 40kDa

Gene ID: 60488

UniProt: P82673