mLC-2 (pThr17) antibody

Catalog No. ABIN7219090

This anti-not_set antibody is a Rabbit Polyclonal antibody detecting not_set in ELISA and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for mLC-2 (pThr17) antibody (ABIN7219090)

Target: mLC-2

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details

Binding Specificity: pThr17

Purpose: MLC-2 (phospho Thr17/S18) Polyclonal Antibody

Specificity: Phospho-MLC-2 (T17/S18) Polyclonal Antibody detects endogenous levels of MLC-2 protein only when phosphorylated at T17/S18.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human MLC-2 Phospho-Thr17/S18

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details

Target: mLC-2

Background: Rabbit Anti-MLC-2 (phospho Thr17/S18) Polyclonal Antibody,MYL12A, MLCB, MRLC3, RLC, Myosin regulatory light chain 12A, MLC-2B, Myosin RLC, Myosin regulatory light chain 2, nonsarcomeric, Myosin regulatory light chain MRLC3, MYL12B, MRLC2, MYLC2B, Myosin regulatory light chain 12B, MLC-2A, MLC-2, M,MYL12A encodes a nonsarcomeric myosin regulatory light chain. Myosin regulatory light chain 12A is activated by phosphorylation and regulates smooth muscle and non-muscle cell contraction. This protein may also be involved in DNA damage repair by sequestering the transcriptional regulator apoptosis-antagonizing transcription factor (AATF)/Che-1 which functions as a repressor of p53-driven apoptosis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 8.,Myosin regulatory light chain 12A

Gene ID: 10627