MMP1 22k antibody (Cleaved-Phe100)

Catalog No. ABIN7212534

The Rabbit Polyclonal anti-MMP1 22k antibody has been validated for WB and ELISA. It is suitable to detect MMP1 22k in samples from Human.

Quick Overview for MMP1 22k antibody (Cleaved-Phe100) (ABIN7212534)

Target: MMP1 22k

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MMP1 22k antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-MMP1 22k Antibody

Binding Specificity: AA 50-130, Cleaved-Phe100

Purpose: Cleaved-MMP-1 22k (F100) Polyclonal Antibody

Specificity: Cleaved-MMP-1 22k (F100) Polyclonal Antibody detects endogenous levels of fragment of activated MMP-1 22k protein resulting from cleavage adjacent to F100.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human MMP-1 22k at AA range: 50-130

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for MMP1 22k

Target: MMP1 22k

Alternative Name: MMP-1 22k

Background: Rabbit Anti-Cleaved-MMP-1 22k (F100) Polyclonal Antibody,MMP1, CLG, Interstitial collagenase, Fibroblast collagenase, Matrix metalloproteinase-1, MMP-1,MMP1 encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This secreted protease (matrix metallopeptidase 1) breaks down the interstitial collagens, including types I, II, and III. MMP1 is part of a cluster of MMP genes on chromosome 11. Mutations in MMP1 are associated with chronic obstructive pulmonary disease (COPD). Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.,Interstitial collagenase

Molecular Weight: observerd band 19kDa

Gene ID: 4312

UniProt: P03956