MEGF9 antibody (AA 360-440)

Catalog No. ABIN7214686

The Rabbit Polyclonal anti-MEGF9 antibody (ABIN7214686) specifically detects MEGF9 in WB and ELISA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for MEGF9 antibody (AA 360-440) (ABIN7214686)

Target: MEGF9 (Multiple EGF-Like-Domains 9 (MEGF9))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MEGF9 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-MEGF9 Antibody

Binding Specificity: AA 360-440

Purpose: EGFL5 Polyclonal Antibody

Specificity: EGFL5 Polyclonal Antibody detects endogenous levels of EGFL5 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human EGFL5 at AA range: 360-440

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for MEGF9

Target: MEGF9 (Multiple EGF-Like-Domains 9 (MEGF9))

Alternative Name: EGFL5

Background: Rabbit Anti-EGFL5 Polyclonal Antibody,MEGF9, EGFL5, KIAA0818, Multiple epidermal growth factor-like domains protein 9, Multiple EGF-like domains protein 9, Epidermal growth factor-like protein 5, EGF-like protein 5,MEGF9 (Multiple EGF-like domains protein 9, also EGF-like protein 5) is a 63 kDa (predicted) novel transmembrane glycoprotein that shares some homology to beta -chains of laminin. It is expressed by hepatocytes, cerebellar Purkinje cells, Schwann cells, keratinocytes and intestinal epithelium. MEGF9 is suggested to participate in cell motility, and its absence correlates with tumor cell migration. Mature human MEGF9 is 572 amino acids (aa) in length. It is a single span type I transmembrane protein that contains a 484 aa extracellular region (aa 31-514) plus a 68 aa C-terminal cytoplasmic domain. The extracellular region possesses a lengthy Pro-rich region (aa 55-200), followed by five EGF-like domains (aa 204-451). MEGF9 may run at approximately 160 kDa in SDS-PAGE, suggesting either heavy glycosylation or dimerization. Over aa 36-514, human MEGF9 shares 76 % aa sequence identity with mouse MEGF9.,Multiple epidermal growth factor-like domains protein 9

Molecular Weight: observerd band 63kDa

Gene ID: 1955

UniProt: Q9H1U4