MLH1 antibody (AA 410-490)

Catalog No. ABIN7215772

This anti-MLH1 antibody is a Rabbit Polyclonal antibody detecting MLH1 in WB, ELISA and IF. Suitable for Human, Mouse and Rat.

Quick Overview for MLH1 antibody (AA 410-490) (ABIN7215772)

Target: MLH1 (MutL Homolog 1 (MLH1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MLH1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-MLH1 Antibody

Binding Specificity: AA 410-490

Purpose: MLH1 Polyclonal Antibody

Specificity: MLH1 Polyclonal Antibody detects endogenous levels of MLH1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human MLH1 at AA range: 410-490

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IF (1:200-1:1000), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for MLH1

Target: MLH1 (MutL Homolog 1 (MLH1))

Alternative Name: MLH1

Background: Rabbit Anti-MLH1 Polyclonal Antibody,MLH1, COCA2, DNA mismatch repair protein Mlh1, MutL protein homolog 1,MLH1 was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E.coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+phenotype) found in HNPCC. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described, but their full-length natures have not been determined.,DNA mismatch repair protein Mlh1

Molecular Weight: observerd band 85kDa

Gene ID: 4292

UniProt: P40692

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response