NAD-ME antibody (AA 180-260)

Catalog No. ABIN7222420

The Rabbit Polyclonal anti-NAD-ME antibody (ABIN7222420) specifically detects NAD-ME in WB, ELISA and IHC (p). The antibody is reactive with Human samples.

Quick Overview for NAD-ME antibody (AA 180-260) (ABIN7222420)

Target: NAD-ME (NAD Dependent Malate Dehydrogenase (NAD-ME))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NAD-ME antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NAD-ME Antibody

Binding Specificity: AA 180-260

Purpose: ME2 Polyclonal Antibody

Specificity: ME2 Polyclonal Antibody detects endogenous levels of ME2 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human ME2 at AA range: 180-260

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for NAD-ME

Target: NAD-ME (NAD Dependent Malate Dehydrogenase (NAD-ME))

Alternative Name: ME2

Background: Rabbit Anti-ME2 Polyclonal Antibody,ME2, NAD-dependent malic enzyme, mitochondrial, NAD-ME, Malic enzyme 2,ME2 encodes a mitochondrial NAD-dependent malic enzyme, a homotetrameric protein, that catalyzes the oxidative decarboxylation of malate to pyruvate. It had previously been weakly linked to a syndrome known as Friedreich ataxia that has since been shown to be the result of mutation in a completely different gene. Certain single-nucleotide polymorphism haplotypes of this gene have been shown to increase the risk for idiopathic generalized epilepsy. Alternatively spliced transcript variants encoding different isoforms found for this gene.,NAD-dependent malic enzyme mitochondrial

Molecular Weight: observerd band 65kDa

Gene ID: 4200

UniProt: P23368

Pathways: Production of Molecular Mediator of Immune Response