NDC80 antibody (AA 320-400)

Catalog No. ABIN7218976

The Rabbit Polyclonal anti-NDC80 antibody has been validated for WB, ELISA and IF. It is suitable to detect NDC80 in samples from Human and Mouse.

Quick Overview for NDC80 antibody (AA 320-400) (ABIN7218976)

Target: NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NDC80 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-NDC80 Antibody

Binding Specificity: AA 320-400

Purpose: Hec1 Polyclonal Antibody

Specificity: Hec1 Polyclonal Antibody detects endogenous levels of Hec1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human Hec1 at AA range: 320-400

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IF (1:200-1:1000), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for NDC80

Target: NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))

Alternative Name: Hec1

Background: Rabbit Anti-Hec1 Polyclonal Antibody,NDC80, HEC, HEC1, KNTC2, Kinetochore protein NDC80 homolog, Highly expressed in cancer protein, Kinetochore protein Hec1, HsHec1, Kinetochore-associated protein 2, Retinoblastoma-associated protein HEC,NDC80 encodes a component of the NDC80 kinetochore complex. The NDC80, kinetochore complex component consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation.,Kinetochore protein NDC80 homolog

Molecular Weight: observerd band 73kDa

Gene ID: 10403

UniProt: O14777

Pathways: Maintenance of Protein Location