N4BP1 antibody (AA 360-440)

Catalog No. ABIN7215944

This Rabbit Polyclonal antibody specifically detects N4BP1 in WB, ELISA and IHC (p). It exhibits reactivity toward Human.

Quick Overview for N4BP1 antibody (AA 360-440) (ABIN7215944)

Target: N4BP1 (NEDD4 Binding Protein 1 (N4BP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This N4BP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-N4BP1 Antibody

Binding Specificity: AA 360-440

Purpose: N4BP1 Polyclonal Antibody

Specificity: N4BP1 Polyclonal Antibody detects endogenous levels of N4BP1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human N4BP1 at AA range: 360-440

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for N4BP1

Target: N4BP1 (NEDD4 Binding Protein 1 (N4BP1))

Alternative Name: N4BP1

Background: Rabbit Anti-N4BP1 Polyclonal Antibody,N4BP1, KIAA0615, NEDD4-binding protein 1, N4BP1,N4BP1 is a Protein Coding gene. NEDD4 Binding Protein 1 is a inhibitor of the E3 ubiquitin-protein ligase ITCH. Acts by interacting with the second WW domain of ITCH, leading to compete with ITCHs substrates and impairing ubiquitination of substrates. Gene Ontology (GO) annotations related to this gene include RNA binding. An important paralog of this gene is ZC3H12C.,NEDD4-binding protein 1

Molecular Weight: observerd band 100kDa

Gene ID: 9683

UniProt: O75113