NUB1 antibody (AA 540-620)

Catalog No. ABIN7216142

This anti-NUB1 antibody is a Rabbit Polyclonal antibody detecting NUB1 in WB, ELISA and IHC (p). Suitable for Human, Mouse and Monkey.

Quick Overview for NUB1 antibody (AA 540-620) (ABIN7216142)

Target: NUB1 (Negative Regulator of Ubiquitin-Like Proteins 1 (NUB1))

Reactivity: Human, Mouse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NUB1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NUB1 Antibody

Binding Specificity: AA 540-620

Purpose: NUB1 Polyclonal Antibody

Specificity: NUB1 Polyclonal Antibody detects endogenous levels of NUB1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the C-terminal region of human NUB1 at AA range: 540-620

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for NUB1

Target: NUB1 (Negative Regulator of Ubiquitin-Like Proteins 1 (NUB1))

Alternative Name: NUB1

Background: Rabbit Anti-NUB1 Polyclonal Antibody,NUB1, NYREN18, NEDD8 µLtimate buster 1, Negative regulator of ubiquitin-like proteins 1, Renal carcinoma antigen NY-REN-18,NUB1 encodes a protein that functions as a negative regulator of NEDD8, a ubiquitin-like protein that conjugates with cullin family members in order to regulate vital biological events. Negative regulator of ubiquitin like proteins 1 encoded by NUB1 regulates the NEDD8 conjugation system post-transcriptionally by recruiting NEDD8 and its conjugates to the proteasome for degradation. Negative regulator of ubiquitin like proteins 1 interacts with the product of the AIPL1 gene, which is associated with Leber congenital amaurosis, an inherited retinopathy, and mutations in that gene can abolish interaction with negative regulator of ubiquitin like proteins 1, which may contribute to the pathogenesis. Negative regulator of ubiquitin like proteins 1 is also known to accumulate in Lewy bodies in Parkinson's disease and dementia with Lewy bodies, and in glial cytoplasmic inclusions in multiple system atrophy, with this abnormal accumulation being specific to alpha-synucleinopathy lesions. Alternative splicing of NUB1 results in multiple transcript variants.,NEDD8 µLtimate buster 1

Molecular Weight: observerd band 70kDa

Gene ID: 51667

UniProt: Q9Y5A7