NOLC1 antibody

Catalog No. ABIN7223956

The Rabbit Polyclonal anti-NOLC1 antibody has been validated for ELISA and IF. It is suitable to detect NOLC1 in samples from Human, Mouse and Rat.

Quick Overview for NOLC1 antibody (ABIN7223956)

Target: NOLC1 (Nucleolar and Coiled-Body Phosphoprotein 1 (NOLC1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOLC1 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF)

Product Details anti-NOLC1 Antibody

Purpose: p130 Polyclonal Antibody

Specificity: P130 Polyclonal Antibody detects endogenous levels of p130 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human p130 around the non-phosphorylation site of S952

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IF (1:200-1:1000), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for NOLC1

Target: NOLC1 (Nucleolar and Coiled-Body Phosphoprotein 1 (NOLC1))

Alternative Name: p130

Background: Rabbit Anti-p130 Polyclonal Antibody,RBL2, RB2, Retinoblastoma-like protein 2, 130 kDa retinoblastoma-associated protein, p130, Retinoblastoma-related protein 2, RBR-2, pRb2,The retinoblastoma (Rb) tumor suppressor family includes the retinoblastoma protein Rb (p105), retinoblastoma-like protein 1 (RBL1, p107), and retinoblastoma-like protein 2 (RBL2, p130). These Rb family proteins are referred to as 'pocket proteins' because they contain a conserved binding pocket region that interacts with critical regulatory proteins, including E2F family transcription factors, c-Abl tyrosine kinase, and proteins containing a conserved LXCXE motif. In quiescent G0 phase cells, active Rb proteins are hypophosphorylated and bind to E2F transcription factors to repress transcription and inhibit cell cycle progression. Upon growth factor induction of quiescent cells, Rb proteins become hyperphosphorylated and inactivated by G1-phase cyclinD-cdk4/6, G1/S-phase cyclin E-cdk2, and G1/S-phase cyclin A-cdk2 complexes. Hyperphosphorylation of Rb proteins results in a loss of E2F binding and allows for transcriptional activation and cell cycle progression. In addition to regulating the cell cycle, Rb proteins regulate chromosome stability, induction, and maintenance of senescence, apoptosis, cellular differentiation, and angiogenesis. Retinoblastoma-like protein 2 (RBL2, p130) is the most predominant and active Rb family member found in quiescent cells. In these cells, RBL2 interacts with E2F4 and E2F5 to recruit the DP, RB-like, E2F, and MuvB protein (DREAM) complex to E2F target genes to repress transcription of multiple genes required for progression into S phase and mitosis. Hypophosphorylation of RBL2 during cellular senescence is required for maintenance of senescent cells.,Retinoblastoma-like protein 2

Gene ID: 5934

UniProt: Q08999

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling