OPN4 antibody (AA 400-480)

Catalog No. ABIN7215736

This anti-OPN4 antibody is a Rabbit Polyclonal antibody detecting OPN4 in WB, ELISA and IF. Suitable for Human.

Quick Overview for OPN4 antibody (AA 400-480) (ABIN7215736)

Target: OPN4 (Opsin 4 (OPN4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OPN4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-OPN4 Antibody

Binding Specificity: AA 400-480

Purpose: Melanopsin Polyclonal Antibody

Specificity: Melanopsin Polyclonal Antibody detects endogenous levels of Melanopsin protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the C-terminal region of human Melanopsin at AA range: 400-480

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IF (1:200-1:1000), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for OPN4

Target: OPN4 (Opsin 4 (OPN4))

Alternative Name: Melanopsin

Background: Rabbit Anti-Melanopsin Polyclonal Antibody,OPN4, MOP, Melanopsin, Opsin-4,Opsins are members of the guanine nucleotide-binding protein (G protein)-coupled receptor superfamily. OPN4 encodes a photoreceptive opsin protein melanopsin that is expressed within the ganglion and amacrine cell layers of the retina. In mouse, retinal ganglion cell axons expressing this gene projected to the suprachiasmatic nucleus and other brain nuclei involved in circadian photoentrainment. In mouse, this protein is coupled to a transient receptor potential (TRP) ion channel through a G protein signaling pathway and produces a physiologic light response via membrane depolarization and increased intracellular calcium. Melanopsin functions as a sensory photopigment and may also have photoisomerase activity. Experiments with knockout mice indicate that this gene attenuates, but does not abolish, photoentrainment. Alternative splicing results in multiple transcript variants encoding different isoforms.,Melanopsin

Molecular Weight: observerd band 55kDa

Gene ID: 94233

UniProt: Q9UHM6