PBK antibody (pThr9)

Catalog No. ABIN7223546

This Rabbit Polyclonal antibody specifically detects PBK in WB, ELISA and IHC (p). It exhibits reactivity toward Human.

Quick Overview for PBK antibody (pThr9) (ABIN7223546)

Target: PBK (PDZ Binding Kinase (PBK))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PBK antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PBK Antibody

Binding Specificity: pThr9

Purpose: PBK (phospho Thr9) Polyclonal Antibody

Specificity: Phospho-PBK (T9) Polyclonal Antibody detects endogenous levels of PBK protein only when phosphorylated at T9.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human PBK Phospho-Thr9

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for PBK

Target: PBK (PDZ Binding Kinase (PBK))

Alternative Name: PBK

Background: Rabbit Anti-PBK (phospho Thr9) Polyclonal Antibody,PBK, TOPK, Lymphokine-activated killer T-cell-originated protein kinase, Cancer/testis antigen 84, CT84, MAPKK-like protein kinase, Nori-3, PDZ-binding kinase, Spermatogenesis-related protein kinase, SPK, T-LAK cell-originated protein kinas,PBK (PDZ binding kinase) encodes a serine/threonine protein kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. The encoded protein may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. Overexpression of PBK has been implicated in tumorigenesis. Alternative splicing results in multiple transcript variants.,Lymphokine-activated killer T-cell-originated protein kinase

Molecular Weight: observerd band 36kDa

Gene ID: 55872

UniProt: Q96KB5