PGC antibody (Internal Region)

Catalog No. ABIN7223132

The Rabbit Polyclonal anti-PGC antibody has been validated for WB, ELISA and IHC (p). It is suitable to detect PGC in samples from Human.

Quick Overview for PGC antibody (Internal Region) (ABIN7223132)

Target: PGC (Pepsinogen C (PGC))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PGC antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PGC Antibody

Binding Specificity: Internal Region

Purpose: Pepsin C Polyclonal Antibody

Specificity: Pepsin C Polyclonal Antibody detects endogenous levels of Pepsin C protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human Pepsin C

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for PGC

Target: PGC (Pepsinogen C (PGC))

Alternative Name: Pepsin C

Background: Rabbit Anti-Pepsin C Polyclonal Antibody,PGC, Gastricsin, Pepsinogen C,PGC (progastricsin) encodes an aspartic proteinase that belongs to the peptidase family A1. The encoded protein is a digestive enzyme that is produced in the stomach and constitutes a major component of the gastric mucosa. This protein is also secreted into the serum. This protein is synthesized as an inactive zymogen that includes a highly basic prosegment. This enzyme is converted into its active mature form at low pH by sequential cleavage of the prosegment that is carried out by the enzyme itself. Polymorphisms in this gene are associated with susceptibility to gastric cancers. Serum levels of this enzyme are used as a biomarker for certain gastric diseases including Helicobacter pylori related gastritis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 1.,Gastricsin

Molecular Weight: observerd band 42kDa

Gene ID: 5225

UniProt: P20142