PFKL antibody (AA 660-740)

Catalog No. ABIN7216516

This Rabbit Polyclonal antibody specifically detects PFKL in WB, ELISA and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PFKL antibody (AA 660-740) (ABIN7216516)

Target: PFKL (Phosphofructokinase, Liver (PFKL))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PFKL antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PFKL Antibody

Binding Specificity: AA 660-740

Purpose: PFK-B Polyclonal Antibody

Specificity: PFK-B Polyclonal Antibody detects endogenous levels of PFK-B protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the C-terminal region of human PFK-B at AA range: 660-740

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for PFKL

Target: PFKL (Phosphofructokinase, Liver (PFKL))

Alternative Name: PFK-B

Background: Rabbit Anti-PFK-B Polyclonal Antibody,PFKL, 6-phosphofructokinase, liver type, Phosphofructo-1-kinase isozyme B, PFK-B, Phosphofructokinase 1, Phosphohexokinase,PFKL encodes the liver (L) subunit of an enzyme that catalyzes the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate, which is a key step in glucose metabolism (glycolysis). This enzyme is a tetramer that may be composed of different subunits encoded by distinct genes in different tissues. Alternative splicing results in multiple transcript variants.,6-phosphofructokinase liver type

Molecular Weight: observerd band 85kDa

Gene ID: 5211

UniProt: P17858

Pathways: Negative Regulation of Hormone Secretion, Warburg Effect