PFKL antibody (Middle Region)

Catalog No. ABIN2782359

There is 1 publication for this product available. The Rabbit Polyclonal anti-PFKL antibody is suitable to detect PFKL in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep and Zebrafish (Danio rerio). It has been validated for WB.

Quick Overview for PFKL antibody (Middle Region) (ABIN2782359)

Target: PFKL (Phosphofructokinase, Liver (PFKL))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PFKL antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PFKL Antibody

Binding Specificity: Middle Region

Purpose: PFKL Antibody - middle region

Sequence: RTNVLGHLQQ GGAPTPFDRN YGTKLGVKAM LWLSEKLREV YRKGRVFANA

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 86%, Rat: 100%, Sheep: 79%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against PFKL. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PFKL

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8108103

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-PFKL antibody (ABIN2782359)

Spellman, Ahmed, Dubach, Gardiner: "Expression of trisomic proteins in Down syndrome model systems." in: Gene, Vol. 512, Issue 2, pp. 219-25, (2012) (PubMed).

Target Details for PFKL

Target: PFKL (Phosphofructokinase, Liver (PFKL))

Alternative Name: PFKL

Background:

Background Information: Phosphofructokinase (PFK) is a tetrameric enzyme that catalyzes a key step in glycolysis, namely the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate. PFK from muscle is a homotetramer of M subunit, PFK from liver is a homotetramer of L-subunits, while PFK from platelets can be composed of any tetrameric combination of M and L subunits. PFKL represents the L subunit.

Gene Name: Phosphofructokinase, liver

Alternative Symbols: PFK-B, PFK-L, ATP-PFK

Protein Name: ATP-dependent 6-phosphofructokinase, liver type

Molecular Weight: 64kDa

Gene ID: 5211

UniProt: P17858

Pathways: Negative Regulation of Hormone Secretion, Warburg Effect