Phospholipase C gamma 2 antibody (pTyr1217)

Catalog No. ABIN7223184

This Rabbit Polyclonal antibody specifically detects Phospholipase C gamma 2 in WB, ELISA, IF and IHC (p). It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for Phospholipase C gamma 2 antibody (pTyr1217) (ABIN7223184)

Target: Phospholipase C gamma 2 (PLCG2)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Phospholipase C gamma 2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Phospholipase C gamma 2 Antibody

Binding Specificity: pTyr1217

Purpose: PLC γ2 (phospho Tyr1217) Polyclonal Antibody

Specificity: Phospho-PLC γ2 (Y1217) Polyclonal Antibody detects endogenous levels of PLC γ2 protein only when phosphorylated at Y1217.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human PLC gamma2 Phospho-Tyr1217

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Phospholipase C gamma 2

Target: Phospholipase C gamma 2 (PLCG2)

Alternative Name: PLC gamma2

Background: Rabbit Anti-PLC γ2 (phospho Tyr1217) Polyclonal Antibody,PLCG2, 1-phosphatidylinositol 4, 5-bisphosphate phosphodiesterase gamma-2, Phosphoinositide phospholipase C-gamma-2, Phospholipase C-IV, PLC-IV, Phospholipase C-gamma-2, PLC-gamma-2,The protein encoded by PLCG2 (phospholipase C gamma 2) is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1D-myo-inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in PLCG2 have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3.,1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2

Molecular Weight: observerd band 148kDa

Gene ID: 5336

UniProt: P16885

Pathways: RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, Inositol Metabolic Process, Myometrial Relaxation and Contraction, Toll-Like Receptors Cascades, VEGF Signaling, BCR Signaling