PHKB antibody (AA 630-710)

Catalog No. ABIN7223140

This Rabbit Polyclonal antibody specifically detects PHKB in WB and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for PHKB antibody (AA 630-710) (ABIN7223140)

Target: PHKB (Phosphorylase Kinase, beta (PHKB))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHKB antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-PHKB Antibody

Binding Specificity: AA 630-710

Purpose: PHKB Polyclonal Antibody

Specificity: PHKB Polyclonal Antibody detects endogenous levels of PHKB protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human PHKB at AA range: 630-710

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for PHKB

Target: PHKB (Phosphorylase Kinase, beta (PHKB))

Alternative Name: PHKB

Background: Rabbit Anti-PHKB Polyclonal Antibody,PHKB, Phosphorylase b kinase regulatory subunit beta, Phosphorylase kinase subunit beta,Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, encoded by two different genes. The beta subunit is the same in both the muscle and hepatic isoforms, encoded by PHKB (phosphorylase kinase regulatory subunit beta), which is a member of the phosphorylase b kinase regulatory subunit family. The gamma subunit also includes the skeletal muscle and hepatic isoforms, encoded by two different genes. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in PHKB cause glycogen storage disease type 9B, also known as phosphorylase kinase deficiency of liver and muscle. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene. Two pseudogenes have been found on chromosomes 14 and 20, respectively.,Phosphorylase b kinase regulatory subunit beta

Molecular Weight: observerd band 124kDa

Gene ID: 5257

UniProt: Q93100

Pathways: Cellular Glucan Metabolic Process