POLR3K antibody

Catalog No. ABIN7231154

The Rabbit Polyclonal anti-POLR3K antibody is suitable to detect POLR3K in samples from Human and Mouse. It has been validated for WB and ELISA.

Quick Overview for POLR3K antibody (ABIN7231154)

Target: POLR3K (Polymerase (RNA) III (DNA Directed) Polypeptide K, 12.3 KDa (POLR3K))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR3K antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-POLR3K Antibody

Purpose: RPC10 Polyclonal Antibody

Specificity: The antibody detects endogenous levels of RPC10 protein

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen

Immunogen: Synthesized peptide derived from part region of human RPC10 protein

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:5000-1:20000).

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium Azide as preservative and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for POLR3K

Target: POLR3K (Polymerase (RNA) III (DNA Directed) Polypeptide K, 12.3 KDa (POLR3K))

Alternative Name: RPC10

Background: Rabbit Anti-RPC10 Polyclonal Antibody,DNA-directed RNA polymerase III subunit RPC10, DNA-directed RNA polymerase III subunit K, RNA polymerase III 12.5 kDa subunit, RNA polymerase III subunit C11,POLR3K encodes a small essential subunit of RNA polymerase III, the polymerase responsible for synthesizing transfer and small ribosomal RNAs in eukaryotes. The carboxy-terminal domain of this subunit shares a high degree of sequence similarity to the carboxy-terminal domain of an RNA polymerase II elongation factor. This similarity in sequence is supported by functional studies showing that this subunit is required for proper pausing and termination during transcription. Pseudogenes of this gene are found on chromosomes 13 and 17. POLR3K (RNA Polymerase III Subunit K) is a Protein Coding gene. Diseases associated with POLR3K include Spherocytosis, Type 5. Among its related pathways are Pyrimidine metabolism (KEGG) and RNA Polymerase III Transcription Initiation.,RPC10

Molecular Weight: observerd band 11kDa

Gene ID: 51728

UniProt: Q9Y2Y1