PSMA3 antibody (pSer250)

Catalog No. ABIN7223676

The Rabbit Polyclonal anti-PSMA3 antibody is suitable to detect PSMA3 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA and IHC (p).

Quick Overview for PSMA3 antibody (pSer250) (ABIN7223676)

Target: PSMA3 (Proteasome Subunit Alpha Type 3 (PSMA3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PSMA3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PSMA3 Antibody

Binding Specificity: pSer250

Purpose: 20S Proteasome α3 (phospho Ser250) Polyclonal Antibody

Specificity: Phospho-20S Proteasome α3 (S250) Polyclonal Antibody detects endogenous levels of 20S Proteasome α3 protein only when phosphorylated at S250.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human 20S Proteasome alpha3 Phospho-Ser250

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for PSMA3

Target: PSMA3 (Proteasome Subunit Alpha Type 3 (PSMA3))

Alternative Name: 20S Proteasome alpha3

Background: Rabbit Anti-20S Proteasome α3 (phospho Ser250) Polyclonal Antibody,PSMA3, HC8, PSC8, Proteasome subunit alpha type-3, Macropain subunit C8, Multicatalytic endopeptidase complex subunit C8, Proteasome component C8,The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits, 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMA3 (proteasome subunit alpha 3) encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Two alternative transcripts encoding different isoforms have been identified.,Proteasome subunit alpha type-3

Gene ID: 5684

UniProt: P25788

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA