PKC antibody (pThr497)

Catalog No. ABIN7223496

This Rabbit Polyclonal antibody specifically detects PKC in WB, ELISA, IF and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PKC antibody (pThr497) (ABIN7223496)

Target: PKC (PKC pan) (Protein Kinase C (pan) (PKC pan))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)

Product Details anti-PKC Antibody

Binding Specificity: pThr497

Purpose: Rabbit Anti-PKC (phospho Thr497) Polyclonal Antibody

Specificity: Phospho-PKC (T497) Polyclonal Antibody detects endogenous levels of PKC protein only when phosphorylated at T497.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human PKC Phospho-Thr497

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:40000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PKC

Target: PKC (PKC pan) (Protein Kinase C (pan) (PKC pan))

Alternative Name: PKC

Background: PRKCA, PKCA, PRKACA, Protein kinase C alpha type, PKC-A, PKC-alpha, PRKCB, PKCB, PRKCB1, Protein kinase C beta type, PKC-B, PKC-beta, PRKCD, Protein kinase C delta type, Tyrosine-protein kinase PRKCD, nPKC-delta, PRKCE, PKCE, Protein kinaseProtein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by PRKCA (protein kinase C alpha) is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.

Molecular Weight: 83kD

Gene ID: 5578, 5579, 5580

UniProt: P17252, P05771, Q05655, Q02156, P05129, P24723, Q04759, Q05513

Pathways: Positive Regulation of Response to DNA Damage Stimulus