PRKAB1 antibody (pSer182)

Catalog No. ABIN7212864

This anti-PRKAB1 antibody is a Rabbit Polyclonal antibody detecting PRKAB1 in WB, ELISA and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for PRKAB1 antibody (pSer182) (ABIN7212864)

Target: PRKAB1 (Protein Kinase, AMP-Activated, beta 1 Non-Catalytic Subunit (PRKAB1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKAB1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PRKAB1 Antibody

Binding Specificity: pSer182

Purpose: AMPKβ1 (phospho Ser182) Polyclonal Antibody

Specificity: Phospho-AMPKβ1 (S182) Polyclonal Antibody detects endogenous levels of AMPKβ1 protein only when phosphorylated at S182.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human AMPKbeta1 Phospho-Ser182

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for PRKAB1

Target: PRKAB1 (Protein Kinase, AMP-Activated, beta 1 Non-Catalytic Subunit (PRKAB1))

Alternative Name: AMPKbeta1

Background: Rabbit Anti-AMPKβ1 (phospho Ser182) Polyclonal Antibody,PRKAB1, AMPK, 5'-AMP-activated protein kinase subunit beta-1, AMPK subunit beta-1, AMPKb,Protein kinase AMP-activated non-catalytic subunit beta 1encoded by PRKAB1 is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex.,5'-AMP-activated protein kinase subunit beta-1

Gene ID: 5564

UniProt: Q9Y478

Pathways: AMPK Signaling, Warburg Effect