PPP1R8 antibody (AA 170-250)

Catalog No. ABIN7223382

The Rabbit Polyclonal anti-PPP1R8 antibody has been validated for WB and ELISA. It is suitable to detect PPP1R8 in samples from Human, Mouse and Rat.

Quick Overview for PPP1R8 antibody (AA 170-250) (ABIN7223382)

Target: PPP1R8 (Protein Phosphatase 1, Regulatory Subunit 8 (PPP1R8))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPP1R8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-PPP1R8 Antibody

Binding Specificity: AA 170-250

Purpose: NIPP1 Polyclonal Antibody

Specificity: NIPP1 Polyclonal Antibody detects endogenous levels of NIPP1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human NIPP1 at AA range: 170-250

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for PPP1R8

Target: PPP1R8 (Protein Phosphatase 1, Regulatory Subunit 8 (PPP1R8))

Alternative Name: NIPP1

Background: Rabbit Anti-NIPP1 Polyclonal Antibody,PPP1R8, ARD1, NIPP1, Nuclear inhibitor of protein phosphatase 1, NIPP-1, Protein phosphatase 1 regulatory inhibitor subunit 8,PPP1R8 (protein phosphatase 1 regulatory subunit 8), through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by PPP1R8 are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA.,Nuclear inhibitor of protein phosphatase 1

Molecular Weight: observerd band 40kDa

Gene ID: 5511

UniProt: Q12972