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PTK6 antibody (pTyr447)

This Rabbit Polyclonal antibody specifically detects PTK6 in WB, ELISA and IF. It exhibits reactivity toward Human.
Catalog No. ABIN7223792
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$322.23
$379.10
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Quick Overview for PTK6 antibody (pTyr447) (ABIN7223792)

Target

See all PTK6 Antibodies
PTK6 (PTK6 Protein tyrosine Kinase 6 (PTK6))

Reactivity

  • 92
  • 30
  • 3
  • 2
Human

Host

  • 82
  • 9
Rabbit

Clonality

  • 84
  • 8
Polyclonal

Conjugate

  • 36
  • 6
  • 6
  • 5
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
This PTK6 antibody is un-conjugated

Application

  • 66
  • 39
  • 26
  • 26
  • 15
  • 14
  • 13
  • 10
  • 6
  • 3
  • 2
  • 2
  • 1
Western Blotting (WB), ELISA, Immunofluorescence (IF)
  • Binding Specificity

    • 24
    • 15
    • 8
    • 8
    • 7
    • 5
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pTyr447

    Purpose

    Brk (phospho Tyr447) Polyclonal Antibody

    Specificity

    Phospho-Brk (Y447) Polyclonal Antibody detects endogenous levels of Brk protein only when phosphorylated at Y447.

    Purification

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

    Immunogen

    Synthesized peptide derived from human Brk Phospho-Tyr447

    Isotype

    IgG
  • Application Notes

    Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IF (1:200-1:1000), ELISA (1:5000). Not yet tested in other applications.

    Comment

    Primary Antibody

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    -20 °C

    Storage Comment

    Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
  • Target

    PTK6 (PTK6 Protein tyrosine Kinase 6 (PTK6))

    Alternative Name

    Brk

    Background

    Rabbit Anti-Brk (phospho Tyr447) Polyclonal Antibody,PTK6, BRK, Protein-tyrosine kinase 6, Breast tumor kinase, Tyrosine-protein kinase BRK,The protein encoded by PTK6 (protein tyrosine kinase 6) is a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Overexpression of PTK6 in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Expression of PTK6 has been detected at low levels in some breast tumors but not in normal breast tissue. The encoded protein has been shown to undergo autophosphorylation. Alternative splicing results in multiple transcript variants.,Protein-tyrosine kinase 6

    Molecular Weight

    observerd band 50kDa

    Gene ID

    5753

    UniProt

    Q13882
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