PC antibody (AA 330-410)

Catalog No. ABIN7222954

The Rabbit Polyclonal anti-PC antibody has been validated for WB, ELISA and IHC (p). It is suitable to detect PC in samples from Human, Mouse and Rat.

Quick Overview for PC antibody (AA 330-410) (ABIN7222954)

Target: PC (Pyruvate Carboxylase (PC))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PC antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PC Antibody

Binding Specificity: AA 330-410

Purpose: PCB Polyclonal Antibody

Specificity: PCB Polyclonal Antibody detects endogenous levels of PCB protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human PCB at AA range: 330-410

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for PC

Target: PC (Pyruvate Carboxylase (PC))

Alternative Name: PCB

Background: Rabbit Anti-PCB Polyclonal Antibody,PC, Pyruvate carboxylase, mitochondrial, Pyruvic carboxylase, PCB,PC encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene.,Pyruvate carboxylase mitochondrial

Molecular Weight: observerd band 120kDa

Gene ID: 5091

UniProt: P11498