RagA/B (AA 240-320) antibody

Catalog No. ABIN7216770

This Rabbit Polyclonal antibody specifically detects RagA/B in IHC, WB, ELISA and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for RagA/B (AA 240-320) antibody (ABIN7216770)

Target: RagA/B

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details

Binding Specificity: AA 240-320

Purpose: Rabbit Anti-Rag A/B Polyclonal Antibody

Specificity: Rag A/B Polyclonal Antibody detects endogenous levels of Rag A/B protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human Rag A/B at AA range: 240-320

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details

Target: RagA/B

Alternative Name: Rag A/B

Background: RRAGA, Ras-related GTP-binding protein A, Rag A, RagA, Adenovirus E3 14.7 kDa-interacting protein 1, FIP-1, RRAGB, Ras-related GTP-binding protein B, Rag B, RagBHas guanine nucleotide-binding activity but undetectable intrinsic GTPase activity. Required for the amino acid-induced relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the TOR signaling cascade by amino acids. Involved in the RCC1/Ran-GTPase pathway. May play a direct role in a TNF-alpha signaling pathway leading to induction of cell death. May alternatively act as a cellular target for adenovirus E3-14. K, an inhibitor of TNF-alpha functions, thereby affecting cell death.

Molecular Weight: 34kD

Gene ID: 10670, 10325

UniProt: Q7L523, Q5VZM2