GRF2 antibody (pTyr504)

Catalog No. ABIN7221290

This Rabbit Polyclonal antibody specifically detects GRF2 in WB, ELISA and IHC (p). It exhibits reactivity toward Human.

Quick Overview for GRF2 antibody (pTyr504) (ABIN7221290)

Target: GRF2 (RAPGEF1) (Rap Guanine Nucleotide Exchange Factor (GEF) 1 (RAPGEF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRF2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GRF2 Antibody

Binding Specificity: pTyr504

Purpose: C3G (phospho Tyr504) Polyclonal Antibody

Specificity: Phospho-C3G (Y504) Polyclonal Antibody detects endogenous levels of C3G protein only when phosphorylated at Y504.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human C3G Phospho-Tyr504

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for GRF2

Target: GRF2 (RAPGEF1) (Rap Guanine Nucleotide Exchange Factor (GEF) 1 (RAPGEF1))

Alternative Name: C3G

Background: Rabbit Anti-C3G (phospho Tyr504) Polyclonal Antibody,RAPGEF1, GRF2, Rap guanine nucleotide exchange factor 1, CRK SH3-binding GNRP, Guanine nucleotide-releasing factor 2, Protein C3G,RAPGEF1 encodes a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK, and activating several members of the Ras family of GTPases. This signaling cascade that may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined.,Rap guanine nucleotide exchange factor 1

Molecular Weight: observerd band 120kDa

Gene ID: 2889

UniProt: Q13905

Pathways: Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Platelet-derived growth Factor Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor