GRF2 antibody (C-Term)

Catalog No. ABIN2785868

The Rabbit Polyclonal anti-GRF2 antibody is suitable to detect GRF2 in samples from Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse and Zebrafish (Danio rerio). It has been validated for WB.

Quick Overview for GRF2 antibody (C-Term) (ABIN2785868)

Target: GRF2 (RAPGEF1) (Rap Guanine Nucleotide Exchange Factor (GEF) 1 (RAPGEF1))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRF2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GRF2 Antibody

Binding Specificity: C-Term

Purpose: RAPGEF1 Antibody - C-terminal region

Sequence: LWAKEQNEEK SPNLTQFTEH FNNMSYWVRS IIMLQEKAQD RERLLLKFIK

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against RAPGEF1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human RAPGEF1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP54753-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for GRF2

Target: GRF2 (RAPGEF1) (Rap Guanine Nucleotide Exchange Factor (GEF) 1 (RAPGEF1))

Alternative Name: RAPGEF1

Background:

Background Information: RAPGEF1 is a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK, and activating several members of the Ras family of GTPases. This signaling cascade protein may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation.This gene encodes a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK, and activating several members of the Ras family of GTPases. This signaling cascade that may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined.

Gene Name: Rap guanine nucleotide exchange factor (GEF) 1

Alternative Symbols: C3G, GRF2

Protein Name: Rap guanine nucleotide exchange factor 1

Molecular Weight: 120kDa

Gene ID: 2889

NCBI Accession: NP_005303

UniProt: Q13905

Pathways: Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Platelet-derived growth Factor Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor