RCC1 antibody (AA 301-350)

Catalog No. ABIN7226940

This Rabbit Polyclonal antibody specifically detects RCC1 in WB, IHC, ELISA and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for RCC1 antibody (AA 301-350) (ABIN7226940)

Target: RCC1 (Regulator of Chromosome Condensation 1 (RCC1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RCC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-RCC1 Antibody

Binding Specificity: AA 301-350

Purpose: Rabbit Anti-RCC1 Polyclonal Antibody

Specificity: The antibody detects endogenous RCC1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthetic peptide from human protein at AA range: 301-350

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,IHC 1:50-200,ELISA 1:10000-20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for RCC1

Target: RCC1 (Regulator of Chromosome Condensation 1 (RCC1))

Alternative Name: RCC1

Background: RCC1, CHC1The Ras family small GTPase Ran is involved in nuclear envelope formation, assembly of the mitotic spindle and nuclear transport. Like other small GTPases, Ran is active in its GTP-bound form and inactive in its GDP-bound form. Nuclear RanGTP concentration is maintained through nuclear localization of guanine nucleotide exchange factor (GEF) activity, which catalyzes the exchange of bound GDP for GTP. RCC1 (regulator of chromatin condensation 1) is the only known RanGEF. RCC1 is dynamically chromatin-bound throughout the cell cycle, and this localization is required for mitosis to proceed normally. Appropriate association of RCC1 with chromatin is regulated through amino-terminal phosphorylation and methylation. RCC1 regulation of RanGTP levels in response to histone modifications regulates nuclear import during apoptosis. In mitosis RCC1 is phosphorylated at Ser11, possibly by cyclinB/cdc2. This phosphorylation may play a role in RCC1 interaction with chromatin and its RanGEF activity.

Molecular Weight: 45kD

Gene ID: 1104

UniProt: P18754

Pathways: Chromatin Binding, Protein targeting to Nucleus