p107 antibody (pThr369)

Catalog No. ABIN7223952

This anti-p107 antibody is a Rabbit Polyclonal antibody detecting p107 in ELISA and IHC (p). Suitable for Human and Mouse.

Quick Overview for p107 antibody (pThr369) (ABIN7223952)

Target: p107 (RBL1) (Retinoblastoma-Like 1 (p107) (RBL1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This p107 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-p107 Antibody

Binding Specificity: pThr369

Purpose: p107 (phospho Thr369) Polyclonal Antibody

Specificity: Phospho-p107 (T369) Polyclonal Antibody detects endogenous levels of p107 protein only when phosphorylated at T369.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human p107 Phospho-Thr369

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC-P (1:100-1:300), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for p107

Target: p107 (RBL1) (Retinoblastoma-Like 1 (p107) (RBL1))

Alternative Name: p107

Background: Rabbit Anti-p107 (phospho Thr369) Polyclonal Antibody,RBL1, Retinoblastoma-like protein 1, 107 kDa retinoblastoma-associated protein, p107, pRb1,The protein encoded by RBL1 (RB transcriptional corepressor like 1) is similar in sequence and possibly function to the product of the retinoblastoma 1 (RB1) gene. The RB1 gene product is a tumor suppressor protein that appears to be involved in cell cycle regulation, as it is phosphorylated in the S to M phase transition and is dephosphorylated in the G1 phase of the cell cycle. Both the RB1 protein and the product of this gene can form a complex with adenovirus E1A protein and SV40 large T-antigen, with the SV40 large T-antigen binding only to the unphosphorylated form of each protein. In addition, both proteins can inhibit the transcription of cell cycle genes containing E2F binding sites in their promoters. Due to the sequence and biochemical similarities with the RB1 protein, it is thought that the protein encoded by this gene may also be a tumor suppressor. Two transcript variants encoding different isoforms have been found for RBL1.,Retinoblastoma-like protein 1

Gene ID: 5933

UniProt: P28749

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases