ARHGDIA antibody (pSer174)

Catalog No. ABIN7222202

This Rabbit Polyclonal antibody specifically detects ARHGDIA in WB, ELISA and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ARHGDIA antibody (pSer174) (ABIN7222202)

Target: ARHGDIA (rho GDP Dissociation Inhibitor (GDI) alpha (ARHGDIA))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARHGDIA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ARHGDIA Antibody

Binding Specificity: pSer174

Purpose: Rho GDIα (phospho Ser174) Polyclonal Antibody

Specificity: Phospho-Rho GDIα (S174) Polyclonal Antibody detects endogenous levels of Rho GDIα protein only when phosphorylated at S174.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Rho GDIalpha Phospho-Ser174

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for ARHGDIA

Target: ARHGDIA (rho GDP Dissociation Inhibitor (GDI) alpha (ARHGDIA))

Alternative Name: Rho GDIalpha

Background: Rabbit Anti-Rho GDIα (phospho Ser174) Polyclonal Antibody,ARHGDIA, GDIA1, Rho GDP-dissociation inhibitor 1, Rho GDI 1, Rho-GDI alpha,ARHGDIA encodes a protein that plays a key role in the regulation of signaling through Rho GTPases. The encoded protein inhibits the disassociation of Rho family members from GDP (guanine diphosphate), thereby maintaining these factors in an inactive state. Activity of this protein is important in a variety of cellular processes, and expression of this gene may be altered in tumors. Mutations in this gene have been found in individuals with nephrotic syndrome, type 8. Alternate splicing results in multiple transcript variants.,Rho GDP-dissociation inhibitor 1

Molecular Weight: observerd band 23kDa

Gene ID: 396

UniProt: P52565

Pathways: Neurotrophin Signaling Pathway