ROCK2 antibody

Catalog No. ABIN7216904

This anti-ROCK2 antibody is a Rabbit Polyclonal antibody detecting ROCK2 in WB and ELISA. Suitable for Human, Mouse, Rat and Monkey.

Quick Overview for ROCK2 antibody (ABIN7216904)

Target: ROCK2 (rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ROCK2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-ROCK2 Antibody

Purpose: Rock-2 Polyclonal Antibody

Specificity: Rock-2 Polyclonal Antibody detects endogenous levels of Rock-2 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Rock-2 around the non-phosphorylation site of Y722

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for ROCK2

Target: ROCK2 (rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2))

Alternative Name: Rock-2

Background: Rabbit Anti-Rock-2 Polyclonal Antibody,ROCK2, KIAA0619, Rho-associated protein kinase 2, Rho kinase 2, Rho-associated, coiled-coil-containing protein kinase 2, Rho-associated, coiled-coil-containing protein kinase II, ROCK-II, p164 ROCK-2,Rho-associated protein kinase 2 encoded by ROCK2 is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.,Rho-associated protein kinase 2

Molecular Weight: observerd band 165kDa

Gene ID: 9475

UniProt: O75116

Pathways: Microtubule Dynamics, WNT Signaling, Tube Formation