RPL5 antibody (AA 130-210)

Catalog No. ABIN7224056

The Rabbit Polyclonal anti-RPL5 antibody has been validated for WB, ELISA and IHC (p). It is suitable to detect RPL5 in samples from Human, Mouse and Rat.

Quick Overview for RPL5 antibody (AA 130-210) (ABIN7224056)

Target: RPL5 (Ribosomal Protein L5 (RPL5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPL5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-RPL5 Antibody

Binding Specificity: AA 130-210

Purpose: Ribosomal Protein L5 Polyclonal Antibody

Specificity: Ribosomal Protein L5 Polyclonal Antibody detects endogenous levels of Ribosomal Protein L5 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human Ribosomal Protein L5 at AA range: 130-210

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for RPL5

Target: RPL5 (Ribosomal Protein L5 (RPL5))

Alternative Name: Ribosomal Protein L5

Background: Rabbit Anti-Ribosomal Protein L5 Polyclonal Antibody,RPL5, MSTP030, 60S ribosomal protein L5,Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL5 (ribosomal protein L5) encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L18P family of ribosomal proteins. It is located in the cytoplasm. The protein binds 5S rRNA to form a stable complex called the 5S ribonucleoprotein particle (RNP), which is necessary for the transport of nonribosome-associated cytoplasmic 5S rRNA to the nucleolus for assembly into ribosomes. The protein interacts specifically with the beta subunit of casein kinase II. Variable expression of RPL5 in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. RPL5 is co-transcribed with the small nucleolar RNA gene U21, which is located in its fifth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.,60S ribosomal protein L5

Molecular Weight: observerd band 34kDa

Gene ID: 6125

UniProt: P46777