RNF111 antibody (AA 870-950)

Catalog No. ABIN7223318

The Rabbit Polyclonal anti-RNF111 antibody is suitable to detect RNF111 in samples from Human and Mouse. It has been validated for WB and ELISA.

Quick Overview for RNF111 antibody (AA 870-950) (ABIN7223318)

Target: RNF111 (Ring Finger Protein 111 (RNF111))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF111 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-RNF111 Antibody

Binding Specificity: AA 870-950

Purpose: Arkadia Polyclonal Antibody

Specificity: Arkadia Polyclonal Antibody detects endogenous levels of Arkadia protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the C-terminal region of human Arkadia at AA range: 870-950

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for RNF111

Target: RNF111 (Ring Finger Protein 111 (RNF111))

Alternative Name: Arkadia

Background: Rabbit Anti-Arkadia Polyclonal Antibody,RNF111, E3 ubiquitin-protein ligase Arkadia, RING finger protein 111,The protein encoded by RNF111 (ring finger protein 111) is a nuclear RING-domain containing E3 ubiquitin ligase. This protein interacts with the transforming growth factor (TGF)-beta/NODAL signaling pathway by promoting the ubiquitination and proteosomal degradation of negative regulators, like SMAD proteins, and thereby enhances TGF-beta target-gene transcription. As a modulator of the nodal signaling cascade, RNF111 plays a critical role in the induction of mesoderm during embryonic development. Alternative splicing of RNF111 results in multiple transcript variants encoding distinct isoforms.,E3 ubiquitin-protein ligase Arkadia

Gene ID: 54778

UniProt: Q6ZNA4