SIAH2 antibody (AA 210-290)

Catalog No. ABIN7217012

The Rabbit Polyclonal anti-SIAH2 antibody (ABIN7217012) specifically detects SIAH2 in WB, ELISA and IHC (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for SIAH2 antibody (AA 210-290) (ABIN7217012)

Target: SIAH2 (Siah E3 Ubiquitin Protein Ligase 2 (SIAH2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIAH2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SIAH2 Antibody

Binding Specificity: AA 210-290

Purpose: Siah-2 Polyclonal Antibody

Specificity: Siah-2 Polyclonal Antibody detects endogenous levels of Siah-2 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the C-terminal region of human Siah-2 at AA range: 210-290

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for SIAH2

Target: SIAH2 (Siah E3 Ubiquitin Protein Ligase 2 (SIAH2))

Alternative Name: Siah-2

Background: Rabbit Anti-Siah-2 Polyclonal Antibody,SIAH2, E3 ubiquitin-protein ligase SIAH2, Seven in absentia homolog 2, Siah-2, hSiah2,SIAH2 encodes E3 ubiquitin-protein ligase SIAH2 that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in regulating cellular response to hypoxia.,E3 ubiquitin-protein ligase SIAH2

Molecular Weight: observerd band 38kDa

Gene ID: 6478

UniProt: O43255