SIRP-Alpha1/beta1 (Internal Region) antibody

Catalog No. ABIN7218750

The Rabbit Polyclonal anti-SIRP-Alpha1/beta1 antibody is suitable to detect SIRP-Alpha1/beta1 in samples from Human. It has been validated for ELISA, IHC (p) and WB.

Quick Overview for SIRP-Alpha1/beta1 (Internal Region) antibody (ABIN7218750)

Target: SIRP-Alpha1/beta1

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Product Details

Binding Specificity: Internal Region

Purpose: SIRP-α1/β1 Polyclonal Antibody

Specificity: SIRP-α1/β1 Polyclonal Antibody detects endogenous levels of SIRP-α1/β1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human SIRP-alpha1/beta1

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details

Target: SIRP-Alpha1/beta1

Background: Rabbit Anti-SIRP-α1/β1 Polyclonal Antibody,SIRPA, BIT, MFR, MYD1, PTPNS1, SHPS1, SIRP, Tyrosine-protein phosphatase non-receptor type substrate 1, SHP substrate 1, SHPS-1, Brain Ig-like molecule with tyrosine-based activation motifs, Bit, CD172 antigen-like family member A, Inhibitory receptor SHP,The signal regulatory protein alpha encoded by SIRPA is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein can be phosphorylated by tyrosine kinases. The phospho-tyrosine residues of this PTP have been shown to recruit SH2 domain containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. This protein was found to participate in signal transduction mediated by various growth factor receptors. CD47 has been demonstrated to be a ligand for this receptor protein. This gene and its product share very high similarity with several other members of the SIRP family. These related genes are located in close proximity to each other on chromosome 20p13. Multiple alternatively spliced transcript variants have been determined for this gene.,Tyrosine-protein phosphatase non-receptor type substrate 1

Molecular Weight: observerd band 55kDa

Gene ID: 140885, 10326, 100653194