Smad2/3 antibody

Catalog No. ABIN7217046

The Rabbit Polyclonal anti-Smad2/3 antibody has been validated for WB, ELISA and IHC (p). It is suitable to detect Smad2/3 in samples from Human, Rat, Mouse and Monkey.

Quick Overview for Smad2/3 antibody (ABIN7217046)

Target: Smad2/3 (SMAD2/3) (Smad2/3 Transcription Factor (SMAD2/3))

Reactivity: Human, Rat, Mouse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Smad2/3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Smad2/3 Antibody

Purpose: Smad2/3 Polyclonal Antibody

Specificity: Smad2/3 Polyclonal Antibody detects endogenous levels of Smad2/3 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Smad2/3 around the non-phosphorylation site of T8

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Smad2/3

Target: Smad2/3 (SMAD2/3) (Smad2/3 Transcription Factor (SMAD2/3))

Alternative Name: Smad2/3

Background: Rabbit Anti-Smad2/3 Polyclonal Antibody,SMAD2, MADH2, MADR2, Mothers against decapentaplegic homolog 2, MAD homolog 2, Mothers against DPP homolog 2, JV18-1, Mad-related protein 2, hMAD-2, SMAD family member 2, SMAD 2, Smad2, hSMAD2, SMAD3, MADH3, Mothers against decapentaplegic,Mothers against decapentaplegic homolog 2 encoded by SMAD2 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF) -beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene.,Mothers against decapentaplegic homolog 2

Molecular Weight: observerd band 48kDa

Gene ID: 4087, 4088