ELK3 antibody (pSer357)

Catalog No. ABIN7220238

This Rabbit Polyclonal antibody specifically detects ELK3 in WB, ELISA, IF and IHC (p). It exhibits reactivity toward Human, Mouse and Monkey.

Quick Overview for ELK3 antibody (pSer357) (ABIN7220238)

Target: ELK3 (ELK3, ETS-Domain Protein (SRF Accessory Protein 2) (ELK3))

Reactivity: Human, Mouse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ELK3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ELK3 Antibody

Binding Specificity: pSer357

Purpose: Net (phospho Ser357) Polyclonal Antibody

Specificity: Phospho-Net (S357) Polyclonal Antibody detects endogenous levels of Net protein only when phosphorylated at S357.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Net Phospho-Ser357

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for ELK3

Target: ELK3 (ELK3, ETS-Domain Protein (SRF Accessory Protein 2) (ELK3))

Alternative Name: Net

Background: Rabbit Anti-Net (phospho Ser357) Polyclonal Antibody,ELK3, NET, SAP2, ETS domain-containing protein Elk-3, ETS-related protein ERP, ETS-related protein NET, Serum response factor accessory protein 2, SAP-2, SRF accessory protein 2,ELK3 encodes a member of the ETS-domain transcription factor family and the ternary complex factor (TCF) subfamily. Proteins in this subfamily regulate transcription when recruited by serum response factor to bind to serum response elements. ELK3, ETS transcription factor is activated by signal-induced phosphorylation, studies in rodents suggest that it is a transcriptional inhibitor in the absence of Ras, but activates transcription when Ras is present. Alternate splicing results in multiple transcript variants.,ETS domain-containing protein Elk-3

Molecular Weight: observerd band 38kDa

Gene ID: 2004

UniProt: P41970