SP1 antibody (pThr453)

Catalog No. ABIN7224432

This anti-SP1 antibody is a Rabbit Polyclonal antibody detecting SP1 in WB, ELISA, IF and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for SP1 antibody (pThr453) (ABIN7224432)

Target: SP1 (Sp1 Transcription Factor (SP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SP1 Antibody

Binding Specificity: pThr453

Purpose: Sp1 (phospho Thr453) Polyclonal Antibody

Specificity: Phospho-Sp1 (T453) Polyclonal Antibody detects endogenous levels of Sp1 protein only when phosphorylated at T453.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Sp1 Phospho-Thr453

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for SP1

Target: SP1 (Sp1 Transcription Factor (SP1))

Alternative Name: Sp1

Background: Rabbit Anti-Sp1 (phospho Thr453) Polyclonal Antibody,SP1, TSFP1, Transcription factor Sp1,The protein encoded by SP1 (Sp1 transcription factor) is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for SP1.,Transcription factor Sp1

Molecular Weight: observerd band 90kDa

Gene ID: 6667

UniProt: P08047

Pathways: Regulation of Lipid Metabolism by PPARalpha, Myometrial Relaxation and Contraction