Sp3/4 antibody (AA 640-720)

Catalog No. ABIN7224438

The Rabbit Polyclonal anti-Sp3/4 antibody has been validated for WB, ELISA, IF and IHC (p). It is suitable to detect Sp3/4 in samples from Human, Mouse and Rat.

Quick Overview for Sp3/4 antibody (AA 640-720) (ABIN7224438)

Target: Sp3/4

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Sp3/4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Sp3/4 Antibody

Binding Specificity: AA 640-720

Purpose: Sp3/4 Polyclonal Antibody

Specificity: Sp3/4 Polyclonal Antibody detects endogenous levels of Sp3/4 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the C-terminal region of human Sp3/4 at AA range: 640-720

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Sp3/4

Target: Sp3/4

Background: Rabbit Anti-Sp3/4 Polyclonal Antibody,SP3, Transcription factor Sp3, SPR-2, SP4, Transcription factor Sp4, SPR-1,SP3 (Sp3 transcription factor) belongs to a family of Sp1 related genes that encode transcription factors that regulate transcription by binding to consensus GC- and GT-box regulatory elements in target genes. This protein contains a zinc finger DNA-binding domain and several transactivation domains, and has been reported to function as a bifunctional transcription factor that either stimulates or represses the transcription of numerous genes. Transcript variants encoding different isoforms have been described for SP3, and one has been reported to initiate translation from a non-AUG (AUA) start codon. Additional isoforms, resulting from the use of alternate downstream translation initiation sites, have also been noted. A related pseudogene has been identified on chromosome 13.,Transcription factor Sp3

Molecular Weight: observerd band 82kDa

Gene ID: 6670, 6671