SPAG8 antibody

Catalog No. ABIN7231482

The Rabbit Polyclonal anti-SPAG8 antibody has been validated for ELISA and WB. It is suitable to detect SPAG8 in samples from Human.

Quick Overview for SPAG8 antibody (ABIN7231482)

Target: SPAG8 (Sperm Associated Antigen 8 (SPAG8))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPAG8 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details anti-SPAG8 Antibody

Purpose: SPAG8 Polyclonal Antibody

Specificity: The antibody detects endogenous levels of SPAG8 protein

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen

Immunogen: Synthesized peptide derived from part region of human SPAG8 protein

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:5000-1:20000).

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium Azide as preservative and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for SPAG8

Target: SPAG8 (Sperm Associated Antigen 8 (SPAG8))

Alternative Name: SPAG8

Background: Rabbit Anti-SPAG8 Polyclonal Antibody,Sperm-associated antigen 8, HSD-1, Sperm membrane protein 1, SMP-1, Sperm membrane protein BS-84,The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. Sperm Associated Antigen 8 encoded by SPAG8 is recognized by sperm agglutinating antibodies from an infertile woman. Sperm Associated Antigen 8 is localized in germ cells of the testis at all stages of spermatogenesis and is localized to the acrosomal region of mature spermatozoa. Sperm Associated Antigen 8 interacts with ACT (activator of CREM in testis) and may play a role in CREM (cAMP response element modulator) -ACT-mediated gene transcription during spermatogenesis. Sperm Associated Antigen 8 may also play a role in spermatogenesis by regulating microtubule formation and cell division. Alternatively spliced variants that encode different protein isoforms have been described but the full-length sequences of only two have been determined.,SPAG8

Molecular Weight: observerd band 46kDa

Gene ID: 26206

UniProt: Q99932